Nisin induction without nisin secretion

Nisin Resistance Protein NSR Proteolytic Degradation of Nisin by the Novel Mechanism for Nisin Resistance via

Novel mechanism for nisin resistance via proteolytic degradation of nisin by the nisin resistance protein NSR.

Nisin is a 34-residue antibacterial peptide produced by Lactococcus lactis that is active against a wide range of gram-positive bacteria. In non-nisin-producing L. lactis, nisin resistance could be conferred by a specific nisin resistance gene (nsr), which encodes a 35-kDa nisin resistance protein (NSR). However, the mechanism underlying NSR-mediated nisin resistance is poorly understood. Here ...

Nisin sensitivity of Bacillus coagulans.

Previous investigations have revealed that Bacillus coagulans strain 43P is sensitive to the polypeptide antibiotic nisin (O'Brien et al., 1956; Campbell and Sniff, 1959; Campbell, Sniff, and O'Brien, 1959). It has also been found that nisin is effective in the control of the flat sour spoilage of tomato juice in inoculated pack studies with this strain of B. coagulans (Campbell et al., 1959). ...

Effects of Tylosin and Nisin

Many antibiotics have been screened for inhibition of sporeforming bacteria during the last 10 years in this laboratory. The effect of some of these antibiotics on canned food spoilage bacteria has previously been described (Denny and Bohrer, 1959). Recently, two antibiotics which eventually may be of use in food processing have been through a preliminary screening in this laboratory. These ant...

Preparation of the antibiotic nisin.

myokinase. A disadvantage of the method is the slow hydrolysis of adenosinetriphosphate in the final stages of the reaction. 2. Metallic salts of adenosinetriphosphate are shown to be unstable and break down on storage to adenylic acid and inorganic pyrophospShate, and to a less extent to adenosinediphosphate and inorganic phosphate. 3. The best preparations of adenosinetriphosphate are at leas...